Action Potential Duration (APD) Purkinje Fiber Assays
Determine the effects of drugs on mammalian cardiac action potentials in vitro
Measurements of concentration-response and rate-dependence of test articles on action potential parameters will be performed: resting membrane potential (RMP, mV), action potential amplitude (APA, mV), maximum rate of rise (Vmax, V/s), & action potential duration at 60 and 90% repolarization (APD60 and APD90, ms). The significance of test article effects will be evaluated versus parallel vehicle and positive control (dl-Sotalol) recordings.
Benefits:
- The APD assay, together with the hERG assay, is an effective indicator of mixed ion channel effects.
- Assays available as a GLP or non-GLP assay conducted on canine or rabbit Purkinje fibers
- Positive control: dl-Sotalol
| Three (3) Concentration Assay Parameters Parameters | GLP | Non-GLP |
|---|---|---|
| Number of concentrations tested | 3 |
3 |
| Number of fibers per test article | 4 |
4 |
| Number of fibers for controls | 4 |
4 |
| Cumulative curves | + |
+ |
| Performed at physiological temperature 37º±1º C | + |
+ |
| Statistics | + |
+ |
| Analytical samples collected | + |
Optional |
| Electronic (pdf) report provided | + |
Optional |
| QAU inspections (in-phase, data, reports) | + |
| One (1) or Two (2) Concentration Screen Assay Parameters Parameters | GLP | Non-GLP |
|---|---|---|
| Number of concentrations tested | 1-2 |
1-2 |
| Number of fibers per test article | 3 |
3 |
| Performed at physiological temperature 37º±1º C | + |
+ |
| Analytical samples collected | + |
|
| Electronic (pdf) report provided | + |
Options:
- Dose Solution Analysis – GLP & non-GLP; verify applied concentrations
- Additional copies of final report (one provided with study)
- Copies of solution worksheets and data-collection forms
- Electronic copy of report (pdf) on CD-ROM
