Functional Ion Channel Assays

hERG Concentration-Response

assay for block of the hERG potassium channel

• manual patch clamp
• 4 concentrations (n = 3)
• vehicle and positive control (n = 3)
• Dose Solution Analysis (DSA) optional
• GLP and non-GLP

Cardiac Channel Panel™

functional assays on all cardiac ion channels

• hERG, Nav1.5, Cav1.2, KvLQT1/minK, Cav3.2, HCN2, HCN4, Kir2.1, Kir3.1/Kir3.4, Kir6.2/SUR2A, Kv1.5, Kv4.3
• 2 concentrations (n=2)
• run on automated patch clamp (PatchXpress®)
• non-GLP only

Examples

Repolarization Assays

SC-hCM APD Assay

highly sensitive assay for changes in action potential duration

• performed on stem cell-derived human cardiomyocytes.
• current clamp patch clamp
• 3 concentrations (n = 3)
• vehicle and positive control (n=2)
• APD60, APD90, APA, Vmax, RMP
• GLP and non-GLP

Alternate Repolarization Assay: Purkinje Fiber (rabbit or canine)

Trafficking Assay

HERG-Lite®

detects hERG expression after prolonged exposures to test article

• 3 concentrations (n = 2)
• non-GLP only

In vivo Cardiovascular Safety Assessment

in vivo canine QT on conscious telemetered beagle dogs

• Subcontracted