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Manual Patch Clamp Recordings. Current traces elicited by a voltage steps from -70 to +70 mV in 10 mV increments, holding potential -80 mV. Horizontal and vertical calibrations are 1 nA and 50 ms, respectively.

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  • Manual Patch Clamp Recordings. Current traces elicited by a voltage steps from -70 to +70 mV in 10 mV increments, holding potential -80 mV. Horizontal and vertical calibrations are 1 nA and 50 ms, respectively.
  • Manual Patch Clamp Recordings. Current-voltage relationship.
  • Manual Patch Clamp Recordings. Currents elicited by +10 mV pulses before (control) and during the application of 1 mM 4-aminopyridine (4-AP).

Human Kv1.6 Potassium Channel EZCells™ TT

  • Cat.#:
  • CT7220

Custom Assay

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Quick Overview

Human Kv1.6 ion channel-expressing, transiently transfected cells.

Background

The human KCNA6 gene encodes the pore-forming subunit of a voltage-gated K+ channel. The hKv1.6 channel expressed in CHO cells displayed outward rectification, activation at depolarized potentials with fast activation and slow inactivation kinetics.
General Info
Product Description Human Kv1.6 ion channel-expressing, transiently transfected cells.
Target Type Ion Channel
Product Type EZCells™ TT
Family Potassium, Voltage-Gated
Sub-Type Kv1.6
Class Voltage-gated potassium channel
Gene Name KCNA6
Validated Assay/Platform manual patch clamp
References Gutman GA, et al. 2005. International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels. Pharmacol Rev. 57:473-508.
EZCell Info
Host cell CHO-K1
Mycoplasma Status Negative (MycoAlert Kit)
Growth media Ham's F12; 10% FBS
Functional expression Mean current amplitude at +10 mV ± SEM = 1.0 ± 0.1 nA (n = 17 cells)
Storage and Packaging
Packaging Cryopreserved, 0.3x106 cells
Storage Recommendation liquid nitrogen
Quantity 0.3x106 cells
Media
Specification Sheet  Download PDF Document