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Background
The human CACNA1A gene encodes the pore-forming subunit of voltage-gated P/Q-type Ca2+ channels. Co-expression with the β4 and α2δ1 auxiliary subunits recapitulates function in heterologous expression (CHO cells). Cav2.1 channels, widely distributed in neurons, are therapeutic targets in treatment of inflammatory pain.
| General Info | |
| Synonyms | P/Q-type calcium channel |
|---|---|
| Product Description | Human Cav2.1/β4/α2δ1 ion channel-expressing, division-arrested cells. |
| Target Type | Ion Channel |
| Product Type | EZCells™ TT |
| Family | Calcium, Voltage-Gated |
| Sub-Type | Cav2.1 |
| Class | Voltage-gated calcium channel |
| Therapeutic Area | pain & inflammation, seizure-convulsion |
| Gene Name | CACNA1A/CACNB4/CACNA2D1 |
| Validated Assay/Platform | manual patch clamp |
| References | Catterall WA et al. 2005. International Union of Pharmacology. XLVIII. Nomenclature and structure-function relationships of voltage-gated calcium channels. Pharmacol Rev. 57:411-25 |
| EZCell Info | |
| Host cell | CHO |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12; 10% FBS |
| Growth characteristics | Cells remain viable 1–2 days post-thaw |
| Functional expression | Mean Ca2+ current amplitude ± SEM at +10 mV= 0.42 ± 0.05 nA, n = 5 (14 additional cells tested had inward current amplitude <200 pA). |
| Storage and Packaging | |
| Packaging | Cryopreserved irradiated cells, 6x106 cells |
| Storage Recommendation | liquid nitrogen |
| Quantity | 6 million cells |
