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Background
| General Info | |
| Product Description | Mouse monoclonal antibody to the TRPV3 cation ion channel. 1 μg/mL was sufficient for detection of TRPV3 in 10μg of COS-1 cell lysate transiently transfected with TRPV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
|---|---|
| Target Type | Ion Channel |
| Product Type | Antibody |
| Family | TRP |
| Sub-Type | TRPV3 |
| Class | Transient Receptor Potential (TRP) |
| Validated Assay/Platform | ICC, IP, WB |
| References | Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. Masamoto Y., Kawabata F., Fushiki T. (2009) Biosci. Biotechnol. Biochem. 73(5): 1021-1027. Xiao R., et al. (2008) J Biol Chem. 283(10): 61 |
| Antibody Info | |
| Immunogen | Synthetic peptide amino acids 458-474 (cytoplasmic C‑terminus) of rat TRPV3 (accession number NP_001 020928) |
| Host and Subclass | Mouse monoclonal, IgG2a |
| Specificity | ~70kDa. |
| Species cross-reactivity | Human, Mouse, Rat. |
| Format | Protein G Purified. In PBS pH7.4, 50% glycerol and 0.09% sodium azide. |
| Recommended Assay Conditions | Concentration and working dilution 1mg/mL; WB: 1-10μg/mL; IHC/ICC: 0.1-1.0μg/mL (Perox), 1.0-10μg/mL (IF) |
| Storage and Packaging | |
| Storage Recommendation | -20°C |
| Stability | 1 year+ |
| Quantity | 100 µg |