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Background
| General Info | |
| Product Description | Mouse monoclonal antibody to the Kv3.1b potassium ion channel. 1 μg/mL was sufficient for detection of Kv3.1b in 10μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
|---|---|
| Target Type | Ion Channel |
| Product Type | Antibody |
| Family | Potassium, Voltage-Gated |
| Sub-Type | Kv3.1b |
| Class | Voltage-gated potassium channel |
| Validated Assay/Platform | IHC, WB |
| References | Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. Xu M., et al. (2007) J. Neuroscience. 27(51): 14158-14170. Zettel M.L., Zhu X., O’Neill W.E., Frisina R.D. (2007) J Assoc Res Otolaryngol. |
| Antibody Info | |
| Immunogen | Fusion protein amino acid 437-585 (cytoplasmic C-Terminus) of rat Kv3.1b (accession number NP_036988) |
| Host and Subclass | Mouse monoclonal, IgG1 |
| Specificity | ~110kDa. |
| Species cross-reactivity | Human (weak), Mouse, Rat. |
| Format | Protein G Purified. In PBS pH7.4, 50% glycerol and 0.09% sodium azide. |
| Recommended Assay Conditions | Concentration and working dilution 1mg/mL; WB: 1-10μg/mL; IHC/ICC: 0.1-1.0μg/mL (Perox), 1.0-10μg/mL (IF) |
| Storage and Packaging | |
| Storage Recommendation | -20°C |
| Stability | 1 year+ |
| Quantity | 100 µg |