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| General Info | |
| Product Description | Mouse monoclonal antibody to the Kir2.2 potassium ion channel. 1 μg/mL was sufficient for detection of Kir2.2 in 10μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
|---|---|
| Target Type | Ion Channel |
| Product Type | Antibody |
| Family | Potassium, Inward Rectifier |
| Sub-Type | Kir2.2 |
| Class | Inward retifier potassium channel |
| Validated Assay/Platform | IHC, WB |
| References | Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. Zobel C., et al. (2003) J Physiol. 550: 365-372. |
| Antibody Info | |
| Immunogen | Fusion protein amino acids 362-427 of rat Kir2.2 (accession number NP_446433) |
| Host and Subclass | Mouse monoclonal, IgG1 |
| Specificity | ~45kDa. No cross reactivity against Kir2.1 or Kir2.3 |
| Species cross-reactivity | Human, Mouse, Rat. |
| Format | Protein G Purified. In PBS pH7.4, 50% glycerol and 0.09% sodium azide. |
| Recommended Assay Conditions | Concentration and working dilution 1mg/mL; WB: 1-10μg/mL; IHC/ICC: 0.1-1.0μg/mL (Perox), 1.0-10μg/mL (IF) |
| Storage and Packaging | |
| Storage Recommendation | -20°C |
| Stability | 1 year+ |
| Quantity | 100 µg |