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Mouse 5HT2b Serotonin Receptor Cell Line

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  • Binding of [3H]-5HT to membranes from cells stably expressing m5HT2b receptor. Membranes from recombinant CHO-K1 cells expressing the m5HT2b receptor were isolated and stored frozen. Radioligand binding studies were performed by incubating membranes with the indicated concentration of [3H]-5HT for 60 min at room temperature, then filtering rapidly over GF/C filters which were then counted in an LSC. Data represent averages of duplicate determinations. 5-HT (10 µM) was used to define non-specific binding.
  • Intracellular calcium response from CHO-K1 cells stably expressing mouse 5HT2b receptors (red) or untransfected controls (black). Cells were loaded with Fluo-4NW then stimulated with the indicated concentrations of α-methylserotonin. Calcium responses were recorded on a FlexStation plate reader. Data represent the average +/- standard deviation of triplicate determinations.

Mouse 5HT2b Serotonin Receptor Cell Line

  • Cat.#:
  • A660
  • Lot #:
  • 736

Availability: In stock

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Quick Overview

Mouse 5HT2b Serotonin Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line.

Background

Serotonin (5-hydroxytryptamine, 5-HT) exerts its many physiological effects through 13 distinct receptor subtypes in humans. These subtypes fall into seven structurally-defined classes (5HT1 to 5HT7). 5HT2b receptors are expressed in the brain, vasculature, smooth muscle, uterus, and stomach. This receptor has been used as a target for developing drugs to treat migraine, anxiety, and irritable bowel syndrome.
General Info
Synonyms HTR2B
Product Description Mouse 5HT2b Serotonin Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line.
Target Type GPCR
Product Type Replicating Cell Line
Receptor Serotonin
Sub-Type 5HT2b
Therapeutic Area cardiac, pulmonary-respiratory
Related Services Membrane Preparation, Large-scale Cryopreservation, GPCR Screening
Genbank Accession Number Amino acid sequence corresponds exactly to BC023690; no expressed tags.
Validated Assay/Platform binding, cAMP
References Choi, D.-S. et al. (1994) The human serotonin 5-HT2B receptor: pharmacological link between 5-HT2 and 5-HT1D receptors. FEBS Lett. 352: 393-399.
Barnes, N.M. (1999) A review of central 5-HT receptors and their function. Neuropharmacology 38: 1
Host cell CHO-K1
Mycoplasma Status Negative (MycoAlert Kit)
Growth media Ham's F12, 10% dFBS, 0.4 mg/mL G418
Storage & Packaging
Packaging Cryopreserved cells, 6 million cells per vial
Storage Recommendation liquid nitrogen
Stability 12 passages (6 weeks of continuous culture)
Quantity 6 million cells
Product Documentation
Specification Sheet  Download PDF Document