||Frozen membranes from CHO-K1 cells
||Membrane Preparation, GPCR Screening
||Cells were harvested and membranes prepared by homogenizing in 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2 and protease inhibitor. The homogenate was centrifuged at 54,000 x g for 10 min at 4o C, and the resulting membranes were washed twice. Membranes were r
|Storage and Packaging|
||1 mL of membrane suspension in storage buffer (50 mM Tris-HCl pH 7.4, 5 mM MgCl2, 10% sucrose) at a protein concentration of 5 mg/mL
||Storage at -80oC is recommended. Repeated freeze-thawing of this product is not recommended.
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