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Background
Protease-activated receptor-2 (PAR2) belongs to a four-member family of GPCRs that are activated by proteases (see Ossovskaya et al for review). These proteases cleave a specific extracellular amino-terminal domain of the receptor to reveal a tethered ligand, which in turn activates the receptor which couples via Gq to release intracellular calcium (Nystedt, S. et al., 1995). PAR2 is expressed in the vasculature, GI tract, CNS, lung, kidney, liver, and heart. PAR2 is a relevant target for arthritis, inflammation, bowel disease, and cancer.
| General Info | |
| Product Description | Frozen membranes from CHO-K1 cells expressing recombinant human PAR2 Protease-Activated receptors. |
|---|---|
| Target Type | GPCR |
| Product Type | membrane |
| Receptor | Protease-Activated |
| Sub-Type | PAR2 |
| Therapeutic Area | cancer, metabolic & gastrointestinal, pain & inflammation |
| Related Services | Membrane Preparation, GPCR Screening |
| Genbank Accession Number | NM_005242 |
| References | Nystedt, S., et al., (1995) Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor 2. Eur. J. Biochem. 232 84-89 Ossovskaya, V.S and Bunnett, N.W. (2004) Protease-activated receptors: contributi |
| Membrane Info | |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Recommended Assay Conditions | Refer to product specification sheet for details |
| Storage and Packaging | |
| Packaging | 0.4 mL of membrane suspension in storage buffer (20 mM HEPES, pH 7.4, 0.1 mM EDTA, 10% sucrose) at a protein concentration of 2 mg/mL. |
| Storage Recommendation | -80°C; Avoid repeated freeze-thawing of this product. |
| Quantity | 400 µL/vial |
| Media | |
| Specification Sheet |  Download PDF Document |
