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Background
The melanocortin 1 receptor is one of the key proteins in regulating hair and skin color. A member of the family of G-protein-coupled receptors, it functions at the surface of pigment producing cells (called melanocytes). After activation by MSH, the MC1 receptor is coupled to Gs signal pathway and is involved in many physical and pathological processes including skin pigmentation, autoimmune disease, inflammation and melanoma.
| General Info | |
| Synonyms | MSHR |
|---|---|
| Product Description | Human MC1 Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Melanocortin |
| Sub-Type | MC1 |
| Therapeutic Area | cancer |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_002386 |
| Validated Assay/Platform | cAMP |
| References | Corn, R.D., et al., The Melanocortin Receptors. Humana Press (2000) Szardenings, M., et al. (1997) Phage Display Selection on Whole Cells Yields a Peptide Specific for Melanocortin Receptor 1. J Biol Chem 272:27943-27948 |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 1x NEAA, 10% dFBS, and 0.5 mg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 12 passages of continuous culture (6 weeks) at 2 passages/week |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human MC1 melanocortin receptor |
| Screening Assay | cAMP assay |
| Readout | Hit Hunter cAMP kit |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 22.59 |
| Z Score | 0.85 |
| DMSO Tolerance | 2.0% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
