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Background
Endothelins are a family of vasoactive peptides that includes ET-1, ET-2 and ET-3. To date, two subtypes of endothelin receptors have been identified from mammalian cells, EDNRA (ETA) and EDNRB (ETB). The EDNRA receptors have a much greater affinity for ET-1 over ET-3 and are expressed abundantly in vascular smooth muscles and stromal tissues. The EDNRB receptors bind ET-1, ET-2 and ET-3 with nearly equal affinity and are expressed abundantly in endothelial cells and epithelial tissues. Although the vasoconstrictor and proliferative effects of ET-1 are primarily mediated by EDNRA receptors, EDNRB receptors can also mediate the vasoconstrictor response through ET-1-induced nitric oxide release from endothelial cells.
| General Info | |
| Synonyms | EDNRB, ETB |
|---|---|
| Product Description | Human ETB Endothelin Receptor-expressing GPCR (G protein-coupled receptor) division-arrested cells. Endothelins are a family of vasoactive peptides that includes ET-1, ET-2 and ET-3. The EDNRA receptors have a much greater affinity for ET-1 over ET-3 and are expressed abundantly in vascular smooth muscles and stromal tissues. The EDNRB receptors bind ET-1, ET-2 and ET-3 with nearly equal affinity and are expressed abundantly in endothelial cells and epithelial tissues. Although the vasoconstrictor and proliferative effects of ET-1 are primarily mediated by EDNRA receptors, EDNRB receptors can also mediate the vasoconstrictor response through ET-1-induced nitric oxide release from endothelial cells. |
| Target Type | GPCR |
| Product Type | EZCells™ DA |
| Receptor | Endothelin |
| Sub-Type | ETB |
| Therapeutic Area | cancer, pulmonary-respiratory, vascular |
| Related Services | Membrane Preparation, GPCR Screening |
| Genbank Accession Number | NM_003991 |
| Validated Assay/Platform | calcium |
| References | Aramori, I., et al., (1992) Coupling of Two Endothelin Receptor Subtypes to Differing Signal Transduction in Transfected Chinese Hamster Ovary Cells. J. Biol. Chem. 267(18): 12468-74. Masaki., et al (1994) International Union of Pharmacology n |
| EZCell Info | |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% dFBS, 1% NEAA, 0.4 mg/mL G418 |
| Storage and Packaging | |
| Packaging | Cryopreserved irradiated cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Quantity | 6 million cells |
| Media | |
| Specification Sheet |  Download PDF Document |
