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Human 5HT1A Serotonin Receptor Cell Line

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  • Binding of [3H] 8-OH-DPAT to membranes from cells stably expressing the human 5 HT1A receptor. Membranes from recombinant CHO-K1 cells expressing the human 5 HT1A receptor were isolated and stored frozen. Radioligand binding studies were performed by incubating membranes with the indicated concentration of [3H] 8-OH-DPAT for 2 hours at 37C in the dark, then filtering rapidly over GF/C filters which were then counted in an LSC. Data represent averages of duplicate determinations. 5-Hydroxytryptamine (10 µM) was used to define non-specific binding (black triangles).
  • 8-OH-DPAT-mediated inhibition of intracellular cAMP accumulation. Cells stably expressing the 5HT1A receptor (green) or untransfected CHO-K1 cells (black) were exposed to the indicated concentrations of 8-OH-DPAT for 30 min at 37oC with 10 µM forskolin and 1 mM IBMX. Intracellular cAMP was assayed using the HitHunter kit (DiscoveRx).

Human 5HT1A Serotonin Receptor Cell Line

  • Cat.#:
  • A601
  • Lot #:
  • 893

Availability: In stock

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Quick Overview

Human 5HT1A Serotonin Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line.

Background

Serotonin (5-hydroxytryptamine, 5-HT) exerts its many physiological effects through 13 distinct receptor subtypes in humans. These subtypes fall into seven structurally-defined classes (5-HT1 to 5-HT7). Among them, 5-HT1a receptors are expressed mainly in the CNS where they induce neuronal inhibition and control behavior, such as sleep, food intake, thermoregulation, aggression, and anxiety. This receptor has been used as a target for developing drugs to treat anxiety and depression.
General Info
Synonyms HTR1A
Product Description Human 5HT1A Serotonin Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line.
Target Type GPCR
Product Type Replicating Cell Line
Receptor Serotonin
Sub-Type 5HT1A
Therapeutic Area neurodegeneration & stroke, psychiatric disorder
Related Services Membrane Preparation, Large-scale Cryopreservation, GPCR Screening
Genbank Accession Number NM_000524
Validated Assay/Platform binding, cAMP
References Raymond, J.R., et al. (1999) The recombinant 5-HT1A receptor: G protein coupling and signaling pathways. Br J Pharmacol. 127(8):1751-64.
Barnes, N.M. (1999) A review of central 5-HT receptors and their function. Neuropharmacology 38: 1083-1152.
Host cell CHO-K1
Mycoplasma Status Negative (MycoAlert Kit)
Growth media Ham's F12, 10% dFBS, 1% NEAA, 400 µg/mL G418
Storage & Packaging
Packaging Cryopreserved cells, 6 million cells per vial
Storage Recommendation liquid nitrogen
Stability 12 passages of continuous culture (6 weeks at 2 passages/week)
Quantity 6 million cells
Product Documentation
Specification Sheet  Download PDF Document