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| General Info | |
| Product Description | Frozen membranes from CHO-K1 cells |
|---|---|
| Target Type | GPCR |
| Product Type | membrane |
| Sub-Type | CHO-K1 |
| Related Services | Membrane Preparation, GPCR Screening |
| Membrane Info | |
| Host cell | CHO-K1 |
| Membrane Preparation | Cells were harvested and membranes prepared by homogenizing in 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2 and protease inhibitor. The homogenate was centrifuged at 54,000 x g for 10 min at 4o C, and the resulting membranes were washed twice. Membranes were r |
| Storage and Packaging | |
| Packaging | 1 mL of membrane suspension in storage buffer (50 mM Tris-HCl pH 7.4, 5 mM MgCl2, 10% sucrose) at a protein concentration of 5 mg/mL |
| Storage Recommendation | Storage at -80oC is recommended. Repeated freeze-thawing of this product is not recommended. |
| Quantity | 1 mL/vial |
| Media | |
| Specification Sheet |  Download PDF Document |
