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Background
Protease-activated receptor-1 (PAR1) belongs to a four-member family of GPCRs that are activated by proteases (see Ossovskaya et al for review). These proteases cleave a specific extracellular amino-terminal domain of the receptor to reveal a tethered ligand, which in turn activates the receptor which couples via Gq to release intracellular calcium (Nystedt, S. et al., 1995). PAR1 is expressed in platelets, vasculature, GI tract, CNS, lung, kidney, liver and heart. PAR1 is a relevant target for coronary thrombosis, inflammatory bowel disease and cancer.
| General Info | |
| Synonyms | F2R. |
|---|---|
| Product Description | Human Protease-Activated PAR1 Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Protease-Activated |
| Sub-Type | PAR1 |
| Therapeutic Area | cancer, neurodegeneration & stroke, vascular |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | BC002464 |
| Validated Assay/Platform | calcium |
| References | Nystedt, S.,et al., (1995) Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor 2. Eur. J. Biochem. 232 84-89 Ossovskaya, V.S and Bunnett, N.W. (2004) Protease-activated receptors: contribution |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, 0.4 mg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | at least 12 passages |
| Quantity | 6 million cells |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
