My Cart
You have no items in your shopping cart.
Background
GPR43 is a member of the free fatty acid G protein-coupled receptor family and is activated by short-chain fatty acids (SCFAs). Expression analysis shows that GPR43 is expressed selectively in leukocytes and to a lesser extent, in spleen. Upon activation by propionate or acetate, GPR43 has been shown to mobilize intracellular calcium in both recombinant systems as well as in human granulocytes.
| General Info | |
| Synonyms | FFAR2 |
|---|---|
| Product Description | Human GPR43 Free Fatty Acid Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Free Fatty Acid |
| Sub-Type | GPR43 |
| Therapeutic Area | metabolic & gastrointestinal |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_005306; no expressed tags |
| Validated Assay/Platform | calcium |
| References | LePoul E., et al. (2003) Functional Characterization of Human Receptors for Short Chain Fatty Acids and Their Role in Polymophonuclear Cell Activation. J. Biol. Chem. 278: 25481-25489 Nilsson, N.E., et al (2003) Identification of a Free Fatty |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, 400 µg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 13 passages (6 weeks of continuous culture) |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human FFAR2 (GPR43) Free Fatty Acid Receptor |
| Screening Assay | calcium mobilization assay |
| Readout | calcium sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 5.78 |
| Z Score | 0.61 |
| DMSO Tolerance | 0.5% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
