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Background
Two receptors for angiotensin II have been identified, AT1 and AT2 receptors. AT1 receptor antagonists have been shown to lower arterial blood pressure, reduce cardiac load, inhibit sympathetic activity and prevent cardiovascular hypertrophy.
| General Info | |
| Synonyms | AT1 |
|---|---|
| Product Description | Human Angiotensin AT1-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Angiotensin |
| Sub-Type | AT1 |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_000685 |
| Validated Assay/Platform | calcium |
| References | Furuta, H., Guo, D.F., et al (1992) Molecular cloning and sequencing of the gene encoding human angiotensin II type 1 receptor. Biochem. Biophys. Res. Commun. 183: 8-13 Takayanagi, R., et al (1992) Molecular cloning, sequence analysis and expr |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, plasmocin 1 ng/mL, 400 µg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | Functional response observed for at least 8 passages |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human AT1 angiotensin receptor |
| Screening Assay | calcium mobilization assay |
| Readout | calcium sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 18.40 |
| Z Score | 0.85 |
| DMSO Tolerance | 0.5% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
