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Background
Serotonin (5-hydroxytryptamine, 5-HT) exerts its many physiological effects through 13 distinct receptor subtypes in humans. These subtypes fall into seven structurally-defined classes (5-HT1 to 5-HT7). 5-HT2B receptors are expressed in the liver, kidney, lung, heart, pancreas, spleen, brain, spinal cord, gastrointestinal tract, placenta, and coronary and pulmonary arteries. It has also been reported in embryonic mouse heart. This receptor has been used as a target for developing drugs to treat migraine, anxiety, and irritable bowel syndrome.
| General Info | |
| Synonyms | HTR2B |
|---|---|
| Product Description | Human Serotonin 5HT2B Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Serotonin |
| Sub-Type | 5HT2B |
| Therapeutic Area | cardiac, pulmonary-respiratory |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_000867 |
| Validated Assay/Platform | calcium |
| References | Choi, D.-S. et al. (1994) The human serotonin 5-HT2B receptor: pharmacological link between 5-HT2 and 5-HT1D receptors. FEBS Lett. 352: 393-399. Barnes, N.M. (1999) A review of central 5-HT receptors and their function. Neuropharmacology 38: 1 |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% dFBS, 1% NEAA, 400 µg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 6 passages (3 weeks of continuous culture) |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human 5HT2b serotonin receptor |
| Screening Assay | calcium mobilization assay |
| Readout | calcium sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 11.60 |
| Z Score | 0.85 |
| DMSO Tolerance | 0.5% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
