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Background
The pituitary hormone vasopressin (AVP) is a cyclic nonapeptide that acts by binding to a family of vasopressin receptors that includes the V1a, V1b, and V2 receptors. V2 receptors are expressed in kidney where vasopressin exerts its antidiuretic action.
| General Info | |
| Product Description | Human V2 Vasopressin Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
|---|---|
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Vasopressin |
| Sub-Type | V2 |
| Therapeutic Area | genitourinary, vascular |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_000054.2 |
| Validated Assay/Platform | binding, calcium |
| References | Lolait, S.J., O’Carroll, A.M., et al., (1992) Cloning and characterization of a vasopressin V2 receptor and possible link to nephrongenic diabetes insipidus. Nature 357: 336-339. Birnbaumer, M., Seibold, A., et al., (1992) Molecular cloning of th |
| Host cell | CHO-K1 |
| Bmax | 19 pmol/mg protein |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, 400 µg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | at least 6 passages |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human V2 vasopressin receptor |
| Screening Assay | calcium mobilization assay |
| Readout | calcium sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 15.90 |
| Z Score | 0.82 |
| DMSO Tolerance | 0.1% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
