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Background
The five muscarinic receptor subtypes all mediate the action of acetylcholine. The odd-numbered receptors (m1, m3, and m5) couple efficiently through Gq/11 to activate phospholipase C and lead to elevation of intracellular calcium, whereas the even-numbered receptors (m2, M4) are efficiently coupled to Gi and lead to inhibition of intracellular cAMP along with several other signal transduction pathways.
| General Info | |
| Product Description | Human M2 Muscarinic Receptor-expressing GPCR (G protein-coupled receptor) division-arrested cells. |
|---|---|
| Target Type | GPCR |
| Product Type | EZCells™ DA |
| Receptor | Muscarinic |
| Sub-Type | m2 |
| Therapeutic Area | cardiac, genitourinary, neurodegeneration & stroke, pulmonary-respiratory |
| Related Services | Membrane Preparation, GPCR Screening |
| Genbank Accession Number | NM_000739 |
| Validated Assay/Platform | binding, cAMP |
| References | Wess, J., Molecular biology of muscarinic acetylcholine receptors. Crit. Rev. Neurobiol., 10: 69-99 (1996). |
| EZCell Info | |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, 400 µg/mL G418 |
| Storage and Packaging | |
| Packaging | Cryopreserved irradiated cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Quantity | 6 million cells |
| Media | |
| Specification Sheet |  Download PDF Document |
