Human FPRL1 Receptor EZCells™

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  • Calcium dose-response for Peptide W stimulation of hFPRL1 EZCells™ compared to non-irradiated cells. CHO-K1 cells that stably express the hFPRL1 receptor (black squares) or similar cells that were irradiated (green triangles) were loaded with Fluo-4 NW, and then stimulated with the indicated concentrations of Peptide W. The resulting increase in intracellular calcium was measured on a FlexStation fluorescent plate reader. Data points represent the average +/- standard deviation of triplicate determinations.

Human FPRL1 Receptor EZCells™

  • Cat.#:
  • A421
  • Lot #:
  • 833

Availability: In stock

Price: $275.00
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Quick Overview

Human FPRL1 Receptor-expressing GPCR (G protein-coupled receptor) division-arrested cells.

Background

The FPR1, FPRL1 and FPRL2 receptors belongs to a family of receptors that all respond to N-formyl peptides. N-Formyl peptides are bacterial peptides which induce chemotaxis in neutrophils and other phagocytic cells to sites of bacterial infection.
General Info
Product Description Human FPRL1 Receptor-expressing GPCR (G protein-coupled receptor) division-arrested cells.
Target Type GPCR
Product Type EZCells™ DA
Receptor Formylpeptide
Sub-Type FPRL1
Therapeutic Area cancer, neurodegeneration & stroke
Related Services Membrane Preparation, GPCR Screening
Genbank Accession Number NM_001462 and Ga16
Validated Assay/Platform binding, calcium
References Murphy, P.M., Ozcelik, T., et al., (1992) A Structural Homologue of the N-Formyl Peptide Receptor. J. Biol. Chem. 267: 7637-7643.
EZCell Info
Host cell CHO-K1
Mycoplasma Status Negative (MycoAlert Kit)
Growth media Ham's F12, 10% FBS, 1% NEAA, 0.4 mg/mL G418, 5 ?g/mL Puromycin
Storage and Packaging
Packaging Cryopreserved irradiated cells, 6 million cells per vial
Storage Recommendation liquid nitrogen
Quantity 6 million cells
Media
Specification Sheet  Download PDF Document