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Background
The FPR1, FPRL1 and FPRL2 receptors belongs to a family of receptors that all respond to N-formyl peptides. N-Formyl peptides are bacterial peptides which induce chemotaxis in neutrophils and other phagocytic cells to sites of bacterial infection.
| General Info | |
| Product Description | Human FPRL1-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
|---|---|
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Formylpeptide |
| Sub-Type | FPRL1 |
| Therapeutic Area | cancer, neurodegeneration & stroke |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_001462 and Ga16 |
| Validated Assay/Platform | binding, calcium |
| References | Murphy, P.M., Ozcelik, T., et al., (1992) A Structural Homologue of the N-Formyl Peptide Receptor. J. Biol. Chem. 267: 7637-7643. |
| Host cell | CHO-K1 |
| Bmax | 1.1 pmol/mg protein |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, 400 µg/mL G418, 5 µg/mL puromycin |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | at least 6 passages |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human FPRL1 N-formyl peptide receptor and Gα16 |
| Screening Assay | calcium mobilization assay |
| Readout | calcium sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 6.60 |
| Z Score | 0.61 |
| DMSO Tolerance | 0.0% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
