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Background
Adenosine is a neuromodulator that is widely distributed in the central and peripheral nervous systems. Many of the actions of adenosine are mediated through G protein-coupled adenosine receptors. Adenosine receptors have been classified into A1, A2A, A2B, and A3 subtypes. Modulation of adenosine receptors by selective agonists and antagonists has potential for the treatment of various cardiovascular inflammatory and neurological diseases.
| General Info | |
| Synonyms | ADORA3 |
|---|---|
| Product Description | Human A3 Adenosine Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Adenosine |
| Sub-Type | A3 |
| Therapeutic Area | cancer, cardiac, neurodegeneration & stroke, pain & inflammation |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_000677 |
| Validated Assay/Platform | cAMP |
| References | Tang, Z., et al. (2007) Polymorphisms in adenosine receptor genes are associated with infarct size in patients with ischemic cardiomyopathy. Clin. Pharmacol. Ther. 82 (4), 435-440 Gao, ZG., et al. (2001) Allosteric Modulation of A3 Adenosine Re |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% dFBS, 1% NEAA, 500 µg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 12 passages of continuous culture (6 weeks at 2 passages/week) |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human A3 adenosine receptor |
| Screening Assay | cAMP assay |
| Readout | Hit Hunter cAMP kit |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 16.75 |
| Z Score | 0.80 |
| DMSO Tolerance | 2.0% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
