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Human ETA Endothelin Receptor EZCells™

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  • Calcium dose-response to endothelin 1 stimulation of EDNRA EZCells™. CHO-K1 cells that stably express the human endothelin receptor type A were either irradiated (green triangles) or were left un-irradiated (black squares). Cells were loaded with Fluo-4NW, and then stimulated with the indicated concentrations of endothelin 1. The resulting increase in intracellular calcium was measured on a FlexStation fluorescent plate reader. Data points represent the average +/- standard deviation of triplicate determinations.

Human ETA Endothelin Receptor EZCells™

  • Cat.#:
  • A492
  • Lot #:
  • 905

Availability: In stock

Price: $275.00
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Quick Overview

Human ETA Endothelin Receptor-expressing GPCR (G protein-coupled receptor) division-arrested cells. Endothelins are a family of vasoactive peptides including ET-1, ET-2 and ET-3 in human. The EDNRA receptors (ETA) have a much greater affinity for ET-1 over ET-3 and are expressed abundantly in vascular smooth muscles and stromal tissues. The vasoconstrictor and proliferative effects of ET-1 are primarily mediated by the EDNRA receptor. This makes EDNRA a good target for drug development in a number of cardiovascular diseases, including congestive heart failure, hypertension, stroke, kidney failure, and a variety of non-cardiovascular conditions, such as asthma, chronic obstructive pulmonary disease, pain and cancer.

Background

Endothelins are a family of vasoactive peptides including ET-1, ET-2 and ET-3 in human. To date, two subtype of endothelin receptors have been identified from mammalian cells, EDNRA (ETA) and EDNRB (ETB). The EDNRA receptors (ETA) have a much greater affinity for ET-1 over ET-3 and are expressed abundantly in vascular smooth muscles and stromal tissues. The vasoconstrictor and proliferative effects of ET-1 are primarily mediated by the EDNRA receptor. This makes EDNRA a good target for drug development in a number of cardiovascular diseases, including congestive heart failure, hypertension, stroke, kidney failure, and a variety of non-cardiovascular conditions, such as asthma, chronic obstructive pulmonary disease, pain and cancer.
General Info
Synonyms EDNRA, ETA
Product Description Human ETA Endothelin Receptor-expressing GPCR (G protein-coupled receptor) division-arrested cells. Endothelins are a family of vasoactive peptides including ET-1, ET-2 and ET-3 in human. The EDNRA receptors (ETA) have a much greater affinity for ET-1 over ET-3 and are expressed abundantly in vascular smooth muscles and stromal tissues. The vasoconstrictor and proliferative effects of ET-1 are primarily mediated by the EDNRA receptor. This makes EDNRA a good target for drug development in a number of cardiovascular diseases, including congestive heart failure, hypertension, stroke, kidney failure, and a variety of non-cardiovascular conditions, such as asthma, chronic obstructive pulmonary disease, pain and cancer.
Target Type GPCR
Product Type EZCells™ DA
Receptor Endothelin
Sub-Type ETA
Therapeutic Area cancer, cardiac, pain & inflammation, pulmonary-respiratory, vascular
Related Services Membrane Preparation, GPCR Screening
Genbank Accession Number NM_001957
Validated Assay/Platform calcium
References Aramori, I., et al., (1992) Couplig of Two Endothelin Receptor Subtypes to Deffering Signal Transduction in Transfected Chinese Hamster Ovary Cells. J Biol chem. 267(18):12468-74.
Masaki., et al (1994) International Union of Pharmacology nomencl
EZCell Info
Host cell CHO-K1
Mycoplasma Status Negative (MycoAlert Kit)
Growth media Ham's F12, 10% dFBS, 1% NEAA, 0.4 mg/mL G418
Storage and Packaging
Packaging Cryopreserved irradiated cells, 6 million cells per vial
Storage Recommendation liquid nitrogen
Quantity 6 million cells
Media
Specification Sheet  Download PDF Document