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Background
The human TRPM4 gene encodes the pore-forming subunit of TRPM4, a non-selective, cation-permeable channel expressed in lymphocytes, heart, vascular smooth muscle, intestine, and prostate. A gain-of-function mutation in TRPM4 is associated with progressive familial heart block type I. TRPM4 channels are therapeutic targets in inflammation and immune disorders.
| General Info | |
| Product Description | Human TRPM4 ion channel-expressing, stable replicating cell line. |
|---|---|
| Target Type | Ion Channel |
| Product Type | Replicating Cell Line |
| Family | TRP |
| Sub-Type | TRPM4 |
| Class | Transient Receptor Potential (TRP) |
| Therapeutic Area | cardiac, pain & inflammation, vascular |
| Related Services | Large-scale Cryopreservation |
| Gene Name | TRPM4 |
| Validated Assay/Platform | FLIPR® |
| References | Clapham DE, et al. 2005. Pharmacol Rev 57: 427 - 450. International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels |
| Host cell | CHO |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 1-2x106 cells |
| Storage Recommendation | liquid nitrogen |
| Quantity | 1-2 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human TRP-M4 TRP channel receptor |
| Screening Assay | membrane potential dye assay |
| Readout | membrane potential sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 14.80 |
| Z Score | 0.81 |
| DMSO Tolerance | 0.2% DMSO |
| Testing Modes | agonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
