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Background
Somatostatin is a cyclic peptide widely expressed in the GI tract, endocrine tissues and brain. It exerts diverse physiological functions including inhibition of release of insulin, glucagon, growth hormone, as well as inhibition of release of pituitary and GI peptides. Five receptors for somatostatin have been identified. This SST4 receptor-expressing cell line is useful for both radioligand binding and for functional assays.
| General Info | |
| Synonyms | SRIF-2 |
|---|---|
| Product Description | Human SSTR4 Somatostatin Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Somatostatin |
| Sub-Type | SSTR4 |
| Therapeutic Area | pain & inflammation, seizure-convulsion |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_001052; no expressed tags |
| Validated Assay/Platform | binding, calcium |
| References | Xu, Y., Song, J. (1993) Molecular cloning and sequencing of a human somatostatin receptor, hSSTR4. Biochem. Biophys. Res. Comun. 193: 648-652. Rohrer, L., Raulf, F. (1993) Cloning and characterization of a fourth human somatostatin receptor. |
| Host cell | CHO-K1 |
| Bmax | 6.8 pmol/mg protein |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 1% NEAA, 400 µg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | at least 8 passages |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human SSTR4 somatostatin receptor |
| Screening Assay | cAMP assay |
| Readout | Hit Hunter cAMP kit |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 26.90 |
| Z Score | 0.82 |
| DMSO Tolerance | 0.25% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
