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Background
Protease-activated receptor-2 (PAR2) belongs to a four-member family of GPCRs that are activated by proteases (see Ossovskaya et al for review). These proteases cleave a specific extracellular amino-terminal domain of the receptor to reveal a tethered ligand, which in turn activates the receptor which couples via Gq to release intracellular calcium (Nystedt, S. et al., 1995). PAR2 is expressed in the vasculature, GI tract, CNS, lung, kidney, liver, and heart. PAR2 is a relevant target for arthritis, inflammation, bowel disease, and cancer.
| General Info | |
| Synonyms | F2RL1, coagulation factor II (thrombin) receptor-like 1. |
|---|---|
| Product Description | Human PAR2 Protease-Activated Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Protease-Activated |
| Sub-Type | PAR2 |
| Therapeutic Area | cancer, metabolic & gastrointestinal, pain & inflammation |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_005242; no expressed tags |
| Validated Assay/Platform | binding, calcium |
| References | Nystedt, S., et al., (1995) Molecular cloning and functional expression of the gene encoding the human proteinase-activated receptor 2. Eur. J. Biochem. 232 84-89 Ossovskaya, V.S and Bunnett, N.W. (2004) Protease-activated receptors: contributi |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | Ham's F12, 10% FBS, 0.4 mg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 12 passages (6 weeks of continuous culture) |
| Quantity | 6 million cells |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
