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Background
Lysophosphatidic acid (LPA) is a small, bioactive phospholipid that mediates multiple cellular responses, including proliferation, differentiation, motility, and survival in both normal physiology and disease. The extracellular actions of LPA are mediated primarily through its interaction with five G protein-coupled receptors which include LPA1(EDG2), LPA2(EDG4), LPA3(EDG7), LPA4 (GPR23 or P2Y9) and LPA5 (GPR92). Among them, LPA1 (EDG2) is most widely expressed in normal tissue during growth and development. After LPA stimulation, LPA1 (EDG2) couples to Gi and G12/13 and was reported to play an important role in development of chronic neuroimmune disease and initiation of neuropathic pain.
| General Info | |
| Synonyms | LPA1, EDG2 |
|---|---|
| Product Description | Human LPA1 Lysophosphatidic Acid Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Lysophosphatidic Acid |
| Sub-Type | LPA1 |
| Therapeutic Area | cancer, pain & inflammation |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | NM_001401 |
| Validated Assay/Platform | binding, cAMP |
| References | Inoue, M., et al., (2004) Initiation of neuropathic pain requires lysophosphatidic acid receptor signaling. Nat. Med. 10(7):712-8. Takuwa, Y., et al (2002) The Edg family G protein-coupled receptors for lysophospholipids: their signaling propert |
| Host cell | RH7777 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | DMEM, 10% FBS, 0.5 mg/mL G418 |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 12 passages of continuous culture (6 weeks) at 2 passages/week |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human LPA1 receptor |
| Screening Assay | cAMP assay |
| Readout | cAMP detection kit |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 10.93 |
| Z Score | 0.64 |
| DMSO Tolerance | 0.5% DMSO |
| Testing Modes | agonist, antagonist, PAM |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
