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Background
GPR120 is a member of the free fatty acid G protein-coupled receptor family that includes GPR40, GPR41 and GPR43, and is activated by unsaturated long chain free fatty acids (Hirasawa et al., 2005). Expression analysis shows that GPR120 is abundantly expressed in intestine. Stimulation of GPR120 by free fatty acids has been shown to promote the secretion of glucagon-like peptide-1 (GLP-1) and activate the ERK cascade (Hirasawa et al., 2005). More recently, Katsuma et al showed that activation of GPR120 by free fatty acids such as linolenic acid inhibits apoptosis of a murine enteroendocrine cell line.
| General Info | |
| Synonyms | GT01, PGR4, GPR129, MGC119984 |
|---|---|
| Product Description | Human GPR120 Free Fatty Acid Receptor-expressing GPCR (G protein-coupled receptor) stable, replicating cell line. |
| Target Type | GPCR |
| Product Type | Replicating Cell Line |
| Receptor | Free Fatty Acid |
| Sub-Type | GPR120 |
| Therapeutic Area | metabolic & gastrointestinal |
| Related Services | Membrane Preparation, Large-scale Cryopreservation, GPCR Screening |
| Genbank Accession Number | BC101175.2 and Ga16; no expressed tags |
| Validated Assay/Platform | calcium |
| References | Hirasawa, A. et al., (2005) Free fatty acids regulate gut incretin glucagon-like peptide-1 secretion through GPR120. Nat. Med. 11: 90-94. Katsuma S. et al. (2005) Free Fatty Acids Inhibit Serum Deprivation-induced Apoptosis through GPR120 in a |
| Host cell | HEK293 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Growth media | DMEM, 10% FBS, 0.4 mg/mL G418, 0.5 mg/mL hygromycin |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 6 million cells per vial |
| Storage Recommendation | liquid nitrogen |
| Stability | 12 passages (6 weeks of continuous culture) |
| Quantity | 6 million cells |
| Representative Screening Information | |
| Cell Line | HEK293 cells stably expressing human GPR120 receptor |
| Screening Assay | calcium mobilization assay |
| Readout | calcium sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 3.40 |
| Z Score | 0.51 |
| DMSO Tolerance | 1.0% DMSO |
| Testing Modes | agonist, antagonist |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
