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Background
The human GABRA1, GABRB2, and GABRG2 genes encode the Cl--selective, ionotropic GABAA receptor-channel. Mutations in GABRA1 have been linked to familial juvenile myoclonic epilepsy. GABAA receptors expressed in CNS neurons are therapeutic targets in treatment seizure and anxiety.
| General Info | |
| Product Description | Human GABAA (a1/b2/g2) Receptor-expressing, stable replicating cell line. |
|---|---|
| Target Type | Ion Channel |
| Product Type | Replicating Cell Line |
| Family | Ligand-Gated |
| Sub-Type | GABAA (α1/β2/γ2) |
| Class | Ligand-gated channel |
| Therapeutic Area | seizure-convulsion |
| Related Services | Large-scale Cryopreservation |
| Gene Name | GABRA1/GABRB2/GABRG2 |
| Validated Assay/Platform | FLIPR® |
| References | Olsen, RW and Sieghart W. 2008. Pharmacol Rev. 60:243-260. International Union of Pharmacology. LXX. Subtypes of g-aminobutyric acidA receptors: Classification on the basis of subunit composition. |
| Host cell | CHO-K1 |
| Mycoplasma Status | Negative (MycoAlert Kit) |
| Storage & Packaging | |
| Packaging | Cryopreserved cells, 1-2x106 cells |
| Storage Recommendation | liquid nitrogen |
| Quantity | 1-2 million cells |
| Representative Screening Information | |
| Cell Line | CHO-K1 stably expressing human GABA-A (α1β2γ2) receptor |
| Screening Assay | membrane potential dye assay |
| Readout | membrane potential sensitive fluorescent dye |
| Plate Format | 384 well |
| Instrument | FlexStation-III |
| Signal Window | 17.99 |
| Z Score | 0.80 |
| DMSO Tolerance | <0.5% DMSO |
| Testing Modes | agonist, antagonist, PAM |
| Testing Options | 16-point minimum, 4-point DRC (n = 4), 8-point DRC (n=3 or 4) |
| Turnaround Time | 2 weeks following receipt of Test Articles |
| Product Documentation | |
| Specification Sheet |  Download PDF Document |
| Screening Info | Download PDF Document |
