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Human beta-2 Adrenergic Receptor Cell Line

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  • Dose response curves of Isoproterenol in ADRB2 cell line. Ratios of two readings obtained prior to and 30 min after the addition of the ligands are plotted (F/F0). EC50 = 12.8 pM in the presence of 25 mM of PDE inhibitor Ro20-1724 and EC50 = 79.9 pM in the absence of PDE inhibitor Ro20-1724
  • EC50 of Isoproterenol is 12.39 nM in the parental cells in the presence of 25 μM of PDE inhibitor Ro20-1724. In the absence of PDE inhibitor, there is not much response to Isoproterenol in the parental cells

Human beta-2 Adrenergic Receptor Cell Line

  • Cat.#:
  • CT80200-253

Availability: In stock

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Quick Overview

Human beta-2 Adrenergic Receptors are stably expressed in GPCR-CNG (G protein-coupled receptor & Cyclic Nucleotide-Gated channel) cells.

Background

These cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently depolarizes cells. Cell depolarization can be easily measured with fluorescent Membrane Potential Sensive Dye (Cat # CT80500-201) using a plate reader or FLIPR and is directly related to intracellular cAMP content.
General Info
Product Description Human beta-2 Adrenergic Receptors are stably expressed in GPCR-CNG (G protein-coupled receptor & Cyclic Nucleotide-Gated channel) cells.
Target Type GPCR
Product Type Replicating Cell Line
Receptor Adrenergic
Sub-Type beta-2
Therapeutic Area pulmonary-respiratory, vascular
Related Services Membrane Preparation, Large-scale Cryopreservation, GPCR Screening
Genbank Accession Number AAA88017.1
Validated Assay/Platform membrane potential
Host cell HEK293-CNG
Mycoplasma Status tested negative for Mycoplasma sp.
Growth media DMEM, 10% FBS, 250 µg/ml G418 and 1 µg/ml puromycin
Storage & Packaging
Packaging Cryopreserved cells, >2.5x106 cells
Storage Recommendation liquid nitrogen
Stability The receptor specific activity is stable for 10 weeks continuous passage
Quantity >2.5 million cells