216.332.1665

Automated Screening Assays:
FLIPRTetra®

Assay Features:

  • 10,000 points per day
  • Uses adherent or non-adherent cell lines
  • Primary cultures of native cells available

Applications:

  • Primary screening of membrane potential, intracellular calcium, potassium (thallium) flux
  • Re-screening

Assays

Intracellular Calcium–signaling by:
  • Calcium channels
    • L & N-type calcium channels in clonal IMR 32 cells
    • L-type calcium channels in clonal HIT cells & in acutely isolated cardiac myocytes
    • Calcium channels in acutely isolated neurons
    • Calcium permeable channels, e.g., TRPs, P2XRs
    • Turnaround time, 1 week
  • G protein-coupled receptors (GPCRs) linked to Gq
Membrane Potential:
  • All recombinant ion channel cell lines with maintained currents
    • Tl+ flux for voltage-sensitive potassium channels
  • G protein-coupled receptors (GPCRs) linked to Gi/o and Gs
    • GPCR-CNG cell-based real time cAMP assay
Customized molecular biology services for GPCRs and Tandem-Pore channels

 

Family Channel
Ligand-gated 5-HT3A
Calcium, voltage-gated Cav3.2 (T-type)
  GABA-A (α1/β2/γ2)
  GABA-A (α2/β2/γ2)
  GABA-A (α5/β2/γ2)
Calcium release activated current ICRAC
Potassium, inward rectifier Kir6.2/SUR2A
Potassium, voltage-gated Kv7.2/Kv7.3 (KCNQ2/KCNQ3)
  Kv7.3/Kv7.5 (KCNQ3/KCNQ5)
  Kv7.4 (KCNQ4)
Sodium, voltage-gated Nav1.5
Sodium/calcium exchanger NCX1
Ligand-gated Nicotinic receptor (nAChR) α3/β4
  Nicotinic receptor (nAChR) α7
  NMDA receptor (NR1/NR2A)
  NMDA receptor (NR1/NR2B)
Purinergic receptors P2X1
  P2X2
  P2X2/P2X3
  P2X3
  P2X4
Potassium, calcium-activated SK2 (KCa2.2)
  SK3 (KCa2.3)
TRP (transient receptor potential) TRPA1
  TRPC6
  TRPM4
  TRPM8
  TRPV1
  TRPV4

 

Download: Ion Channel targets and current platform validation list.

For a quote or additional information, please email inquiries@chantest.com or call 216-332-1665.