Transiently Transfected Cell Line Development
Construction and validation of stable cell lines is very labor-, time-, and cost-intensive. For ion channel cell lines, many functional channels are heteromers of multiple pore-forming and modulatory subunits. Coexpression necessitates the use of multiple antibiotic selection markers often resulting in unhealthy, slow-growing cells. Expression can be lost over time so the critical decision to make an inducible or constitutive expressing-cell line is dependent upon ion channel type.
Transiently transfected cells can be a viable alternative to stable cell line production, but conventional transfection methods are not easily scalable for high-throughput screening applications. ChanTest uses a scalable electroporation system to efficiently deliver cDNA into cells eliminating many of the complications associated with viral vectors or membrane-modifying reagents. With this method, cDNAs encoding principal and auxiliary ion channel or GPCR subunits can be cotransfected to recapitulate functional characteristics.
Transiently Transfected EZCells™ TT
- time and cost savings compared to development of stable cell lines
- multiple subunit expression
- readily scalable for high-throughput screening (HTS)
- high transfection efficiency
- cryopreservation of assay-ready cells
- single, quality-controlled batches for day-to-day assay consistency
To receive a quote or for additional information, please email email@example.com or call 216-332-1665.
For a listing of EZCells™ TT cell lines currently available from ChanTest, click here.