Construction and validation of stable cell lines is very labor-, time-, and cost-intensive. For ion channel cell lines, many functional channels are heteromers of multiple pore-forming and modulatory subunits. Coexpression necessitates the use of multiple antibiotic selection markers often resulting in unhealthy, slow-growing cells. Expression can be lost over time so the critical decision to make an inducible or constitutive expressing-cell line is dependent upon ion channel type.

Transiently transfected cells can be a viable alternative to stable cell line production, but conventional transfection methods are not easily scalable for high-throughput screening applications. ChanTest uses a scalable electroporation system to efficiently deliver cDNA into cells eliminating many of the complications associated with viral vectors or membrane-modifying reagents. With this method, cDNAs encoding principal and auxiliary ion channel or GPCR subunits can be cotransfected to recapitulate functional characteristics.

Transiently Transfected EZCells™ TT

• time and cost savings compared to development of stable cell lines
• multiple subunit expression
• readily scalable for high-throughput screening (HTS)
• high transfection efficiency
• cryopreservation of assay-ready cells
• single, quality-controlled batches for day-to-day assay consistency

To receive a quote or for additional information, please email inquiries@chantest.com or call 216-332-1665.

For a listing of EZCells™ TT cell lines currently available from ChanTest, click here.

Related Services
Stable Cell Line Development
Cryopreservation Services