ChanTest has experience with GPCRs, Ion Channels, promiscuous G proteins, reporter genes, and transporters and has constructed and validated over 70 stable, high-expression, pharmacologically validated cell lines for clients.

Cell Line Development Service - process and options:

• Obtaining and sub-cloning gene into either our own optimized proprietary expression vector or into an expression vector of your choice
• Sequence verification
• DNA preparation and quantitation
• Site-directed mutagenesis, if needed
• Stable transfection into cell line of your choice
• Co-transfection of Gα16, reporter gene or any other necessary or requested component
• Cell selection (G418, hygromycin, puromycin, etc )
• Colony isolation & screening by FACS sorting
• Colony validation by:
  • Intracellular calcium (FlexStation Plate Reader)
  • Membrane potential dyes (FlexStation Plate Reader)
  • Electrophysiological (multiple platforms)
  • Intracellular cAMP determinations
  • Radioligand binding
  • ELISA, Western
• Identify, validate and cryopreserve best and multiple back-up colonies
• Bank best colony
• Test for Mycoplasma prior to shipping
• Send weekly updates and comprehensive final report

Prior to project initiation, we will work with you to tailor a proposal that meets your specific requirements under terms of a confidentiality agreement.

Ordering Information: To discuss requirements, timing and pricing please call 216-332-1665 or email inquiries@chantest.com.

Related Services
Transiently Transfected Cell Line Development
Cryopreservation Services