Manual patch clamp screen

Higher Throughput hERG Block Screen (HTHBS)
Rapid, economical manual-patch screen for hERG risk

Benefits:
  • The HTHBS uses a prescribed protocol (see table below) to provide a quick, low-cost screen of the in vitro effects of 10 or more compounds on the hERG channel

# of Compounds

Concentration

Measurements

Positive Control

Test Conditions

> 10

1 or 10 μM

2

Cisapride

Isochronal measurement (five-minute exposure)
Room temperature


Ion Channel Block Comparator Assays (xBC)
Quickly compare your drug for ion channel block with other drugs in the same class.

Benefits:
  • Compare & rank test articles within a given class of drugs
  • Table of results provided promptly

Cardiac Ion Channel Block Comparator Assays Positive Control
hERG (IKr) Channel Block Comparator Assay (HBC) Cisapride or Terfenadine
Sodium Channel (Nav1.5) Block Comparator Assay (NaBC) Lidocaine
Calcium Channel (Cav1.2 L-type) Block Comparator Assay (CaBC) Nifedipine
Kv1.5 Channel (IKur) Block Comparator Assay (Kv1.5BC) 4-AP
Kv4.3 Channel (IKto) Block Comparator Assay (Kv4.3BC) Flecainide
KvLQT1/minK Channel (IKs) Block Comparator Assay (LQTBC) Chromanol-293B
Ion Channel Screen – Multiple Channel Screen Varies with channel
Assay Parameters Details
Number of concentrations 1 or 2
Number of measurements 3
Positive control (2 measurements) 1 per group
Vehicle control (3 measurements) Available upon request, 1 per group
Additional options
Collection and/or shipment of analytical samples (if requested)

 SAB-Manual patch screen bulletin.pdf