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FLIPRTetra® Screens of Ion Channel Function
HTS: 10,000 points per day
Benefits:
- Turnaround time of 1-2 days
- Uses adherent or non-adherent cell lines
- Primary cultures of native cells available
Applications:
- Primary screening of membrane potential, intracellular calcium, potassium (thallium) flux
- Rescreening
Assays:
| Intracellular Calcium – signaling by: |
- Calcium channels L & N-type calcium channels in clonal IMR 32 cells L-type calcium channels in clonal HIT cells & in acutely isolated cardiac myocytes Calcium channels in acutely isolated neurons Calcium permeable channels, e.g., TRPs, P2XRs Turnaround time, 1 week
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G protein-coupled receptors (GPCRs) linked to Gq
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| Membrane Potential: |
- All recombinant ion channel cell lines with maintained currents Tl+ flux for voltage-sensitive potassium channels
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| Customized molecular biology services for GPCRs and Tandem-Pore channels |
 SAB-FLIPR screens.pdf
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