FAST & Lite screen

FAST & Lite
Combining FASTPatch and HERG-Lite assays,
FAST & Lite is a comprehensive screening for both defective trafficking and direct block

FASTPatch
Hundreds of points per day, gigaseals, Raccess < 5MΩ full voltage range,
gating, sensitivity, positive control

HERG-Lite is a chemiluminescent assay that monitors hERG expression at the cell surface. It identifies hERG trafficking inhibitors by exposing a wild-type (WT) hERG to drug overnight. HERG-Lite® identifies hERG channel blockers by measuring rescue of a trafficking-defective mutant hERG (SM) cell line.

Benefits:
  • FASTPatch is fully validated by ChanTest’s expert electrophysiologists
  • Only HERG-Lite detects compounds, such as anti-cancer drugs like arsenic trioxide and geldanamycin derivations that produce hERG risk indirectly by inhibiting trafficking
  • HERG-Lite/TF assay detects trafficking inhibition and mature and immature hERG proteins
  • Identifies compounds that block hERG directly and rescue the hERG SM cell line
  • Results provided 48 hours after study completion

Assay Protocol Positive Control
FASTPatch Profiling Screen Test 1-2 concentrations per compound @ n=2-3, plus positive control per group Tabular results Turnaround time, 1 week 1 concentration @ n=2 per group

Assay # TAs Protocol Concentrations Positive Control Negative Control
HERG-Lite® 10 + 3 conc. @ n=3, on SM & WT, plus pos. & neg. controls per group Turnaround time: 1 week 1, 10 & 30 uM* @ n=3 per conc. *or your choice Astemizole* 1 conc. @ n=3 per group *or your choice DMSO 1 conc. @ n=6 per group
HERG-Lite®/TF 5 + 3 conc. @ n=3, on WT only Turnaround time: 1 week




 SAB-FASTPatch and SFP Bulletin.pdf
 SAB-HERG lite.pdf