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Automated Patch Clamp Screening Assays:
104 Ion Channels & Growing HEK and CHO Hosts
FASTPatch
Hundreds of points per day, gigaseals, Raccess < 5MΩ full voltage range,
gating, sensitivity, positive control
System: PatchXpress® 7000A or QPatch automated patch clamp system
Benefits:
- FASTPatch is fully validated by ChanTest’s expert electrophysiologists
- For safety and discovery, provides IC50s, use-dependence, on-off drug multi-concentration screening for hERG and other channels
| Assay |
Protocol |
Positive Control |
| FASTPatch Proflling Screen |
Test 1-2 concentrations per compound @ n=2-3, plus positive control per group Tabular results Turnaround time, 1 week |
1 concentration @ n=2 per group |
| FASTPatch IC50 Screen |
Test 3-4 concentrations per compound @ n=2-3, plus positive control per group Concentration-response curve & IC50 Turnaround time, 1 week |
1 concentration @ n=2 per group |
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Options:
- FAST & Lite – combines FASTPatch and HERG-Lite
- Comprehensive screening for both defective trafficking and direct block
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Super FASTPatch
2,000 points per day – megohm seals, voltage range, gating, positive control
System: IonWorks Quattro automated patch clamp system
Benefits:
- Super FASTPatch is fully validated by ChanTest’s expert electrophysiologists
- For fast screens, profiles IC50s
| Assay |
Protocol |
Positive Control |
| Super FASTPatch Proflling Screen |
Test 1-8 concentrations per compound @ n=2-3, plus positive control per group Tabular results Turnaround time, 2 days to 1 week |
1 concentration @ n=2 per group |
 SAB-FASTPatch and SFP Bulletin.pdf
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