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Action Potential Duration (APD) Purkinje Fiber Assays
Determine the effects of drugs on mammalian cardiac action potentials in vitro
Measurements of concentration-response and rate-dependence of test articles on action potential parameters will be performed: resting membrane potential (RMP, mV), action potential amplitude (APA, mV), maximum rate of rise (Vmax, V/s), & action potential duration at 60 and 90% repolarization (APD60 and APD90, ms). The significance of test article effects will be evaluated versus parallel vehicle and positive control (dl-Sotalol) recordings.
Benefits:
- The APD assay, together with the hERG assay, is an effective indicator of mixed ion channel effects.
- Assays available as a GLP or non-GLP assay conducted on canine or rabbit Purkinje fibers
- Positive control: dl-Sotalol
Three (3) Concentration Assay Parameters
Parameters |
GLP
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Non-GLP
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| Number of concentrations tested |
3
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3
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| Number of fibers per test article |
4
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4
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| Number of fibers for controls |
4
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4
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| Cumulative curves |
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| Performed at physiological temperature 37º±1º C |
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| Statistics |
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| Analytical samples collected |
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| Electronic (pdf) report provided |
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| QAU inspections (in-phase, data, reports) |
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One (1) or Two (2) Concentration Screen Assay Parameters
Parameters |
GLP
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Non-GLP
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| Number of concentrations tested |
1 or 2
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1 or 2
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| Number of fibers per test article (positive control included) |
3
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3
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| Performed at physiological temperature 37º±1º C |
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| Analytical samples collected |
+
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| Electronic (pdf) report provided |
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Options:
- Dose Solution Analysis – GLP & non-GLP; verify applied concentrations
- Use of non-GLP animals as a protocol exception in GLP assays is available at a lower price
- Additional copies of final report (one provided with study)
- Copies of solution worksheets and data-collection forms
- Electronic copy of report (pdf) on CD-ROM
 CRB-APD assay bulletin.pdf CRB-Rabbit vs canine.pdf
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